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antibodies against stat4  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc antibodies against stat4
    Antibodies Against Stat4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibodies+against+stat4/Stat4+Rabbit+mAb/pm41814359-137-13-18
    Average 95 stars, based on 131 article reviews
    antibodies against stat4 - by Bioz Stars, 2026-10
    95/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: MicroRNA-200a and microRNA-141 have a synergetic effect on the suppression of epithelial-mesenchymal transition in liver cancer by targeting STAT4
    Article Snippet: Proteins (30 μg per lane) were separated by PAGE using 10% Bis-Tris gels (Beyotime Institute of Biotechnology, Inc.) and transferred onto a polyvinylidene fluoride membrane. .. Immunoblotting was performed with diluted antibodies against STAT4 (1:1,000; cat. no. 2653; Cell Signaling Technology, Inc.), E-cadherin (1:1,000; cat. no. 3195; Cell Signaling Technology, Inc.), and vimentin (1:1,000; cat. no. 5741; Cell Signaling Technology, Inc.), and an internal reference antibody against β-actin (1:1,000; cat. no. 4970; Cell Signaling Technology, Inc.) overnight at 4°C. .. The membrane was washed with 0.1% Tween-20 in TBST three times and then incubated with goat anti-rabbit IgG (1:5,000; cat. no. HAF008; R&D Systems, Inc.) for 1 h at room temperature.

    Blocking Assay:

    Article Title: IL-9 signaling redirects CAR T cell fate toward CD8 + memory and CD4 + cycling states, enhancing antitumor efficacy.
    Article Snippet: Article IL-9 signaling redirects CAR T cell fate toward CD8+ memory and CD4+ cycling states, enhancing antitumor efficacy

    Incubation:

    Article Title: IL-9 signaling redirects CAR T cell fate toward CD8 + memory and CD4 + cycling states, enhancing antitumor efficacy.
    Article Snippet: Article IL-9 signaling redirects CAR T cell fate toward CD8+ memory and CD4+ cycling states, enhancing antitumor efficacy

    Article Title: Multi-omics analysis reveals the association of cesarean delivery with altered gut microbial profiles and a Th2-biased immune response in neonates.
    Article Snippet: Equal amounts of protein were mixed with 5× loading buffer (Cat# C05-03001, Bioss, USA), denatured by heating at 95–100 °C for 5–10 min, and separated on 10% SDS-PAGE gels (Cat# SPGT001, Biolight, China). .. After electrophoresis, proteins were transferred to a membrane, blocked, and probed with primary antibodies against STAT4 (Cat# 2653T, CST, USA) and STAT6 (Cat# 5397T, CST, USA), followed by incubation with appropriate HRP-conjugated secondary antibodies. .. Protein bands were detected using ECL substrate (Cat# C05-07004, Bioss,

    Article Title: Integrated transcriptomics and epigenomics reveal chamber-specific and species-specific characteristics of human and mouse hearts
    Article Snippet: The protein samples were separated by 4% to 12% SDS-PAGE gels (Invitrogen, Carlsbad, California, USA, Cat. NP0323BOX) followed by electrotransfer to polyvinylidene fluoride (PVDF) membranes (Merck Millipore, Burlington, Massachusetts, USA, Cat. ISEQ00010). .. The membranes were blocked in 5% milk in Tris-buffered saline (TBS)-Tween (Applygen, Beijing, China, Cat. B1009) for 1 h at room temperature and then incubated overnight at 4°C in primary antibodies against STAT4 (CST, Danvers, Massachusetts, USA, Cat. 2653, 1:1,000), ANKRD2 (Proteintech, Rosemont, Illinois, USA, Cat. 11821, 1:1,000), or GAPDH (Proteintech, Cat. 60004, 1:20,000). .. After the membranes were washed 5 times in TBS-T for 5 min at room temperature, they were incubated with secondary antibodies conjugated to HRP (ZSGB-BIO, Beijing, China, Cat. ZB-2301, Cat. ZB-2305) for 1 h at room temperature.

    Electrophoresis:

    Article Title: Multi-omics analysis reveals the association of cesarean delivery with altered gut microbial profiles and a Th2-biased immune response in neonates.
    Article Snippet: Equal amounts of protein were mixed with 5× loading buffer (Cat# C05-03001, Bioss, USA), denatured by heating at 95–100 °C for 5–10 min, and separated on 10% SDS-PAGE gels (Cat# SPGT001, Biolight, China). .. After electrophoresis, proteins were transferred to a membrane, blocked, and probed with primary antibodies against STAT4 (Cat# 2653T, CST, USA) and STAT6 (Cat# 5397T, CST, USA), followed by incubation with appropriate HRP-conjugated secondary antibodies. .. Protein bands were detected using ECL substrate (Cat# C05-07004, Bioss,

    Membrane:

    Article Title: Multi-omics analysis reveals the association of cesarean delivery with altered gut microbial profiles and a Th2-biased immune response in neonates.
    Article Snippet: Equal amounts of protein were mixed with 5× loading buffer (Cat# C05-03001, Bioss, USA), denatured by heating at 95–100 °C for 5–10 min, and separated on 10% SDS-PAGE gels (Cat# SPGT001, Biolight, China). .. After electrophoresis, proteins were transferred to a membrane, blocked, and probed with primary antibodies against STAT4 (Cat# 2653T, CST, USA) and STAT6 (Cat# 5397T, CST, USA), followed by incubation with appropriate HRP-conjugated secondary antibodies. .. Protein bands were detected using ECL substrate (Cat# C05-07004, Bioss,

    Saline:

    Article Title: Integrated transcriptomics and epigenomics reveal chamber-specific and species-specific characteristics of human and mouse hearts
    Article Snippet: The protein samples were separated by 4% to 12% SDS-PAGE gels (Invitrogen, Carlsbad, California, USA, Cat. NP0323BOX) followed by electrotransfer to polyvinylidene fluoride (PVDF) membranes (Merck Millipore, Burlington, Massachusetts, USA, Cat. ISEQ00010). .. The membranes were blocked in 5% milk in Tris-buffered saline (TBS)-Tween (Applygen, Beijing, China, Cat. B1009) for 1 h at room temperature and then incubated overnight at 4°C in primary antibodies against STAT4 (CST, Danvers, Massachusetts, USA, Cat. 2653, 1:1,000), ANKRD2 (Proteintech, Rosemont, Illinois, USA, Cat. 11821, 1:1,000), or GAPDH (Proteintech, Cat. 60004, 1:20,000). .. After the membranes were washed 5 times in TBS-T for 5 min at room temperature, they were incubated with secondary antibodies conjugated to HRP (ZSGB-BIO, Beijing, China, Cat. ZB-2301, Cat. ZB-2305) for 1 h at room temperature.

    other:

    Article Title: Quantitative analysis of JAK/STAT signaling pathway in patients of inflammatory skin disorders.
    Article Snippet: Background Inflammatory skin diseases (ISDs), are characterized by dysregulated activation of innate and adaptive immune systems, with inflammatory cytokines playing a crucial role in their pathogenesis.. Objectives This study aimed to investigate the involvement of Janus kinase/signal transduction and activator of transcription (JAK/STAT) signaling pathway in the pathogenesis of ISDs.. Methods The study analyzed a total of 117 skin biopsies, comprising 31 from pyoderma gangrenosum (PG), 25 from hidradenitis suppurativa (HS), 35 from psoriasis patients, and 26 from control subjects.

    Article Title: BaP/BPDE exposure causes human trophoblast cell dysfunctions and induces miscarriage by up-regulating lnc-HZ06-regulated IL1B.
    Article Snippet: Exposure to environmental BaP or its metabolite BPDE causes trophoblast cell dysfunctions to induce miscarriage (abnormal early embryo loss), which might be generally regulated by lncRNAs.. IL1B, a critical inflammatory cytokine, is closely associated with adverse pregnancy outcomes.. However, whether IL1B might cause dysfunctions of BaP/BPDE-exposed trophoblast cells to induce miscarriage, as well as its specific epigenetic regulatory mechanisms, is completely unexplored.

    Immunoprecipitation:

    Article Title: Lkb1 maintains T reg cell lineage identity
    Article Snippet: Cell lysis was prepared using Co-immunoprecipitation kits according to the manufacturer’s protocol (Sigma, FLAGITP1), and incubated with antibodies against Dnmt1 (60B1220.1, Abcam), Dnmt3a (Abcam) and protein G agarose (Cell Signalling Technology). .. Immunoprecipitated proteins were detected as described above with antibodies against STAT4 (C46B10, Cell Signalling Technology) and STAT5 (Cell Signalling Technology). .. ChIP assays were performed using the ChIP kits (Active Motif, 53040) according to the manufacturer’s protocol.



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    BMSC-CM activated fibroblasts by activating the <t>JAK2/STAT4</t> pathway. ( A ) The expression of JAK2, p-JAK2, STAT4, and p-STAT4 in fibroblasts treated with different media was determined by western blotting. ( B ) The expression of three most significant DEGs ( POSTN , COMP , and TGFBI ) in fibroblasts treated with JAK2 inhibitor AG490 (10 μM). ( C ) Wound–scratch assay in fibroblasts treated with or without JAK2 inhibitor AG490 (10 μM) at 0 h, 24 h, and 48 h (magnification, ×40). Scale bar = 400 μm. ( D ) The percentage of migration area in different groups. ( E ) The CCK-8 assay showing reduced proliferation of fibroblasts treated with BMSC-CM after treatment with AG490. ( F ) Collagen synthesis in fibroblasts after AG490 treatment was determined by RT-PCR. Data are shown as means ± standard deviation (SD). * P < 0.05; ** P < 0.01; *** P < 0.001. BMSC-CM, bone marrow stem cell-conditioned medium; con, control medium treatment group; CM, BMSC-CM treatment group.
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    BMSC-CM activated fibroblasts by activating the <t>JAK2/STAT4</t> pathway. ( A ) The expression of JAK2, p-JAK2, STAT4, and p-STAT4 in fibroblasts treated with different media was determined by western blotting. ( B ) The expression of three most significant DEGs ( POSTN , COMP , and TGFBI ) in fibroblasts treated with JAK2 inhibitor AG490 (10 μM). ( C ) Wound–scratch assay in fibroblasts treated with or without JAK2 inhibitor AG490 (10 μM) at 0 h, 24 h, and 48 h (magnification, ×40). Scale bar = 400 μm. ( D ) The percentage of migration area in different groups. ( E ) The CCK-8 assay showing reduced proliferation of fibroblasts treated with BMSC-CM after treatment with AG490. ( F ) Collagen synthesis in fibroblasts after AG490 treatment was determined by RT-PCR. Data are shown as means ± standard deviation (SD). * P < 0.05; ** P < 0.01; *** P < 0.001. BMSC-CM, bone marrow stem cell-conditioned medium; con, control medium treatment group; CM, BMSC-CM treatment group.
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    Immunoexpression of JAK/STAT proteins in the epidermis, normal skin, × 400. a Immunoexpression of JAK3 in the epidermis, normal skin, 10.73 ± 3.36. b Immunoexpression of STAT2 in the epidermis, normal skin, 11.06 ± 5.34. c Immunoexpression of <t>STAT4</t> in the epidermis, normal skin, 18.59 ± 3.01. d Immunoexpression of STAT6 in the epidermis, normal skin, 11.56 ± 2.84
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    Image Search Results


    BMSC-CM activated fibroblasts by activating the JAK2/STAT4 pathway. ( A ) The expression of JAK2, p-JAK2, STAT4, and p-STAT4 in fibroblasts treated with different media was determined by western blotting. ( B ) The expression of three most significant DEGs ( POSTN , COMP , and TGFBI ) in fibroblasts treated with JAK2 inhibitor AG490 (10 μM). ( C ) Wound–scratch assay in fibroblasts treated with or without JAK2 inhibitor AG490 (10 μM) at 0 h, 24 h, and 48 h (magnification, ×40). Scale bar = 400 μm. ( D ) The percentage of migration area in different groups. ( E ) The CCK-8 assay showing reduced proliferation of fibroblasts treated with BMSC-CM after treatment with AG490. ( F ) Collagen synthesis in fibroblasts after AG490 treatment was determined by RT-PCR. Data are shown as means ± standard deviation (SD). * P < 0.05; ** P < 0.01; *** P < 0.001. BMSC-CM, bone marrow stem cell-conditioned medium; con, control medium treatment group; CM, BMSC-CM treatment group.

    Journal: Aging (Albany NY)

    Article Title: Bone marrow stem cells secretome accelerates simulated birth trauma-induced stress urinary incontinence recovery in rats

    doi: 10.18632/aging.202812

    Figure Lengend Snippet: BMSC-CM activated fibroblasts by activating the JAK2/STAT4 pathway. ( A ) The expression of JAK2, p-JAK2, STAT4, and p-STAT4 in fibroblasts treated with different media was determined by western blotting. ( B ) The expression of three most significant DEGs ( POSTN , COMP , and TGFBI ) in fibroblasts treated with JAK2 inhibitor AG490 (10 μM). ( C ) Wound–scratch assay in fibroblasts treated with or without JAK2 inhibitor AG490 (10 μM) at 0 h, 24 h, and 48 h (magnification, ×40). Scale bar = 400 μm. ( D ) The percentage of migration area in different groups. ( E ) The CCK-8 assay showing reduced proliferation of fibroblasts treated with BMSC-CM after treatment with AG490. ( F ) Collagen synthesis in fibroblasts after AG490 treatment was determined by RT-PCR. Data are shown as means ± standard deviation (SD). * P < 0.05; ** P < 0.01; *** P < 0.001. BMSC-CM, bone marrow stem cell-conditioned medium; con, control medium treatment group; CM, BMSC-CM treatment group.

    Article Snippet: The above-mentioned steps were performed and antibodies against JAK2 (1:1000, CST, 3230S, USA), p-JAK2 (1:1000, CST, 4406S, USA), STAT4 (1:1000, HuaBio, ET1701-42, China) and p-STAT4 (1:1000, Affinity Biosciences, AF3441, 1:1000) were used.

    Techniques: Expressing, Western Blot, Wound Healing Assay, Migration, CCK-8 Assay, Reverse Transcription Polymerase Chain Reaction, Standard Deviation

    BMSC-CM activated fibroblasts by activating the JAK2/STAT4 pathway. ( A ) The expression of JAK2, p-JAK2, STAT4, and p-STAT4 in fibroblasts treated with different media was determined by western blotting. ( B ) The expression of three most significant DEGs ( POSTN , COMP , and TGFBI ) in fibroblasts treated with JAK2 inhibitor AG490 (10 μM). ( C ) Wound–scratch assay in fibroblasts treated with or without JAK2 inhibitor AG490 (10 μM) at 0 h, 24 h, and 48 h (magnification, ×40). Scale bar = 400 μm. ( D ) The percentage of migration area in different groups. ( E ) The CCK-8 assay showing reduced proliferation of fibroblasts treated with BMSC-CM after treatment with AG490. ( F ) Collagen synthesis in fibroblasts after AG490 treatment was determined by RT-PCR. Data are shown as means ± standard deviation (SD). * P < 0.05; ** P < 0.01; *** P < 0.001. BMSC-CM, bone marrow stem cell-conditioned medium; con, control medium treatment group; CM, BMSC-CM treatment group.

    Journal: Aging (Albany NY)

    Article Title: Bone marrow stem cells secretome accelerates simulated birth trauma-induced stress urinary incontinence recovery in rats

    doi: 10.18632/aging.202812

    Figure Lengend Snippet: BMSC-CM activated fibroblasts by activating the JAK2/STAT4 pathway. ( A ) The expression of JAK2, p-JAK2, STAT4, and p-STAT4 in fibroblasts treated with different media was determined by western blotting. ( B ) The expression of three most significant DEGs ( POSTN , COMP , and TGFBI ) in fibroblasts treated with JAK2 inhibitor AG490 (10 μM). ( C ) Wound–scratch assay in fibroblasts treated with or without JAK2 inhibitor AG490 (10 μM) at 0 h, 24 h, and 48 h (magnification, ×40). Scale bar = 400 μm. ( D ) The percentage of migration area in different groups. ( E ) The CCK-8 assay showing reduced proliferation of fibroblasts treated with BMSC-CM after treatment with AG490. ( F ) Collagen synthesis in fibroblasts after AG490 treatment was determined by RT-PCR. Data are shown as means ± standard deviation (SD). * P < 0.05; ** P < 0.01; *** P < 0.001. BMSC-CM, bone marrow stem cell-conditioned medium; con, control medium treatment group; CM, BMSC-CM treatment group.

    Article Snippet: The above-mentioned steps were performed and antibodies against JAK2 (1:1000, CST, 3230S, USA), p-JAK2 (1:1000, CST, 4406S, USA), STAT4 (1:1000, HuaBio, ET1701-42, China) and p-STAT4 (1:1000, Affinity Biosciences, AF3441, 1:1000) were used.

    Techniques: Expressing, Western Blot, Wound Healing Assay, Migration, CCK-8 Assay, Reverse Transcription Polymerase Chain Reaction, Standard Deviation

    Immunoexpression of JAK/STAT proteins in the epidermis, normal skin, × 400. a Immunoexpression of JAK3 in the epidermis, normal skin, 10.73 ± 3.36. b Immunoexpression of STAT2 in the epidermis, normal skin, 11.06 ± 5.34. c Immunoexpression of STAT4 in the epidermis, normal skin, 18.59 ± 3.01. d Immunoexpression of STAT6 in the epidermis, normal skin, 11.56 ± 2.84

    Journal: Immunologic Research

    Article Title: Expression of JAK3, STAT2, STAT4, and STAT6 in pemphigus vulgaris

    doi: 10.1007/s12026-020-09122-y

    Figure Lengend Snippet: Immunoexpression of JAK/STAT proteins in the epidermis, normal skin, × 400. a Immunoexpression of JAK3 in the epidermis, normal skin, 10.73 ± 3.36. b Immunoexpression of STAT2 in the epidermis, normal skin, 11.06 ± 5.34. c Immunoexpression of STAT4 in the epidermis, normal skin, 18.59 ± 3.01. d Immunoexpression of STAT6 in the epidermis, normal skin, 11.56 ± 2.84

    Article Snippet: Endogenous peroxidase activity was blocked by 0.3% hydrogen peroxide in distilled water and then, sections were rinsed with Tris-buffered saline (TBS, Dako, Denmark), and incubated with primary rabbit polyclonal antibody against STAT2 (Santa Cruz Biotechnology Inc.), mouse monoclonal antibody against STAT4 (Santa Cruz Biotechnology, Inc.), and primary rabbit polyclonal antibody against STAT6 (Santa Cruz biotechnology Inc.) and incubated overnight with mouse monoclonal antibody against JAK3.

    Techniques:

    Immunoexpression of JAK/STAT proteins in the epidermis, pemphigus vulgaris, × 400. Immunoexpression of JAK3 in the epidermis: a perilesional skin 14.38 ± 3.61; b skin lesions 18.89 ± 4.67, p > 0.05. Immunoexpression of STAT2 in the epidermis: c perilesional skin 15.79 ± 2.06; d skin lesions 17.15 ± 2.81, non-significant. Immunoexpression of STAT4 in the epidermis: e perilesional skin 24.10 ± 3.40; f skin lesions 29.08 ± 4.38, p < 0.05. Immunoexpression of STAT6 in the epidermis: g perilesional skin 18.21 ± 3.49; h skin lesions 27.85 ± 4.68, p < 0.05

    Journal: Immunologic Research

    Article Title: Expression of JAK3, STAT2, STAT4, and STAT6 in pemphigus vulgaris

    doi: 10.1007/s12026-020-09122-y

    Figure Lengend Snippet: Immunoexpression of JAK/STAT proteins in the epidermis, pemphigus vulgaris, × 400. Immunoexpression of JAK3 in the epidermis: a perilesional skin 14.38 ± 3.61; b skin lesions 18.89 ± 4.67, p > 0.05. Immunoexpression of STAT2 in the epidermis: c perilesional skin 15.79 ± 2.06; d skin lesions 17.15 ± 2.81, non-significant. Immunoexpression of STAT4 in the epidermis: e perilesional skin 24.10 ± 3.40; f skin lesions 29.08 ± 4.38, p < 0.05. Immunoexpression of STAT6 in the epidermis: g perilesional skin 18.21 ± 3.49; h skin lesions 27.85 ± 4.68, p < 0.05

    Article Snippet: Endogenous peroxidase activity was blocked by 0.3% hydrogen peroxide in distilled water and then, sections were rinsed with Tris-buffered saline (TBS, Dako, Denmark), and incubated with primary rabbit polyclonal antibody against STAT2 (Santa Cruz Biotechnology Inc.), mouse monoclonal antibody against STAT4 (Santa Cruz Biotechnology, Inc.), and primary rabbit polyclonal antibody against STAT6 (Santa Cruz biotechnology Inc.) and incubated overnight with mouse monoclonal antibody against JAK3.

    Techniques:

    Morphometric analysis of JAK3 (upper left), STAT2 (upper right), STAT4 (lower left), and STAT6 (lower right) immunoexpression in keratinocytes. The results of semiquantitative analysis are expressed as the mean ± standard deviation. Control – normal skin. PV-P – pemphigus vulgaris perilesional skin, PV-L – pemphigus vulgaris skin lesions. The level of significance is defined where p < 0.05. JAK3: C vs. PV-L ( p < 0.05); PV-P vs. PV-L ( p < 0.05); STAT2: C vs. PV-L ( p < 0.05); STAT4: C vs. PV-P (NS), C vs. PV-L (NS); STAT6: C vs. PV-P ( p < 0.05), C vs. PV-L ( p < 0.05), PV-P vs. PV-L ( p < 0.05)

    Journal: Immunologic Research

    Article Title: Expression of JAK3, STAT2, STAT4, and STAT6 in pemphigus vulgaris

    doi: 10.1007/s12026-020-09122-y

    Figure Lengend Snippet: Morphometric analysis of JAK3 (upper left), STAT2 (upper right), STAT4 (lower left), and STAT6 (lower right) immunoexpression in keratinocytes. The results of semiquantitative analysis are expressed as the mean ± standard deviation. Control – normal skin. PV-P – pemphigus vulgaris perilesional skin, PV-L – pemphigus vulgaris skin lesions. The level of significance is defined where p < 0.05. JAK3: C vs. PV-L ( p < 0.05); PV-P vs. PV-L ( p < 0.05); STAT2: C vs. PV-L ( p < 0.05); STAT4: C vs. PV-P (NS), C vs. PV-L (NS); STAT6: C vs. PV-P ( p < 0.05), C vs. PV-L ( p < 0.05), PV-P vs. PV-L ( p < 0.05)

    Article Snippet: Endogenous peroxidase activity was blocked by 0.3% hydrogen peroxide in distilled water and then, sections were rinsed with Tris-buffered saline (TBS, Dako, Denmark), and incubated with primary rabbit polyclonal antibody against STAT2 (Santa Cruz Biotechnology Inc.), mouse monoclonal antibody against STAT4 (Santa Cruz Biotechnology, Inc.), and primary rabbit polyclonal antibody against STAT6 (Santa Cruz biotechnology Inc.) and incubated overnight with mouse monoclonal antibody against JAK3.

    Techniques: Standard Deviation, Control